Journal: Oncology Letters

Article Title: ZAP70 interaction with 13 mRNAs as a potential immunotherapeutic target for endometrial cancer

doi: 10.3892/ol.2023.13799

Figure Lengend Snippet: In vivo and in vitro validation of ZAP70 expression and its potential role in prognosis. (A) ZAP70 mRNA expression was detected in tissues by reverse transcription-quantitative PCR. (B) ZAP70 protein expression was detected in tissues by immunohistochemistry. Image intake was presented through the microscope (cat. no. 703548; Nikon Corporation). (C) Localization of ZAP70 in cells was detected using immunofluorescence (400×). Image intake was presented through the microscope (cat. no. 703548; Nikon Corporation). Differences in ZAP70 between (D) healthy and tumoral tissues, (E) FIGO clinical stages I/II and stages III/IV, and (F) living and deceased patients. IHC, immunohistochemistry; OS, overall survival; TPM, transcripts per kilobase million; ZAP70, ζ-chain-associated protein kinase 70. *P<0.05 and ***P<0.001.

Article Snippet: The 2-step plus Poly-HRP Anti Rabbit/Mouse IgG Detection System immunohistochemistry kit (cat. no. E-IR-R211; Elabscience Biotechnology, Inc.) was used to analyze tissue sections (4 μm).

Techniques: In Vivo, In Vitro, Biomarker Discovery, Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction, Immunohistochemistry, Microscopy, Immunofluorescence

Journal: Marine Drugs

Article Title: Pyropia yezoensis Protein Supplementation Prevents Dexamethasone-Induced Muscle Atrophy in C57BL/6 Mice

doi: 10.3390/md16090328

Figure Lengend Snippet: Effects of PYCP supplementation on IGF-IR and IRS-1 phosphorylation in gastrocnemius muscle. The protein levels of p-IGF-IR, IGF-IR, p-IRS-1, and IRS-1 were assessed by Western blot analysis. GAPDH was used as an internal standard. Data represent mean ± standard deviation of three independent experiments (n = 5/group). ** p < 0.01 vs. the corresponding control group; ## p < 0.01 vs. the corresponding DEX-only treatment group.

Article Snippet: p-IGF-IR , Santa Cruz Biotechnology: sc-101703 , Rabbit , 1:1000.

Techniques: Phospho-proteomics, Western Blot, Standard Deviation, Control

Journal: Marine Drugs

Article Title: Pyropia yezoensis Protein Supplementation Prevents Dexamethasone-Induced Muscle Atrophy in C57BL/6 Mice

doi: 10.3390/md16090328

Figure Lengend Snippet: Primary antibodies (monoclonal or polyclonal) used in the western blot analysis.

Article Snippet: p-IGF-IR , Santa Cruz Biotechnology: sc-101703 , Rabbit , 1:1000.

Techniques: Western Blot